Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors

Publication Type:

Journal Article

Source:

Angewandte Chemie International Edition, Volume 55, Issue 40, p.12440–12444 (2016)

URL:

http://doi.wiley.com/10.1002/anie.201606123http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fanie.201606123

Abstract:

The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations.

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