Disease detection by ultrasensitive quantification of microdosed synthetic urinary biomarkers.

Publication Type:

Journal Article


J Am Chem Soc, Volume 136, Issue 39, p.13709-14 (2014)


The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting, and produce urinary reporters of disease state. We establish a customized single molecule detection assay that enables urinary discrimination of thromboembolic disease in mice using doses of the nanoparticulate diagnostic agents that fall under regulatory guidelines for microdosing.