Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors
Publication Type:
Journal Article
Authors:
Jain, Piyush K.; Ramanan, Vyas; Schepers, Arnout G.; Dalvie, Nisha S.; Panda, Apekshya; Fleming, Heather E.; Bhatia, Sangeeta N.
Source:
Angewandte Chemie International Edition, Volume 55, Issue 40, p.12440–12444 (2016)
URL:
Abstract:
The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations.
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