Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes
Publication Type:
Journal Article
Authors:
Piyush K Jain, Justin H Lo, Santosh Rananaware, Marco Downing, Apekshya Panda, Michelle Tai, Srivatsan Raghavan, Heather E Fleming, and Sangeeta N Bhatia.
Source:
Nanoscale (2019)
URL:
http://pubs.rsc.org/en/content/articlepdf/2019/NR/C9NR01786K
Abstract:
There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9 to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and completely based on biologically-derived materials could offer such potential. Here we report a simple and modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficiently combines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/Cas9 ribonucleoprotein complexes to yield robust genome editing across multiple cell lines.
