Disease detection by ultrasensitive quantification of microdosed synthetic urinary biomarkers.

Written By Melodi Anahtar

PUBLICATION TYPE:

Journal Article

AUTHORS:

Andrew D Warren†; Shonda T Gaylord†; Ngan, Kevin C; Dumont Milutinovic, Milena; Kwong, Gabriel A; Sangeeta N Bhatia‡; David R Walt‡

SOURCE:

J Am Chem Soc, Volume 136, Issue 39, p.13709-14 (2014)

URL:

http://www.ncbi.nlm.nih.gov/pubmed/25198059?dopt=Abstract

ABSTRACT:

The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting, and produce urinary reporters of disease state. We establish a customized single molecule detection assay that enables urinary discrimination of thromboembolic disease in mice using doses of the nanoparticulate diagnostic agents that fall under regulatory guidelines for "microdosing."

Manuscript + SI (PDF)

Melodi Anahtar
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